The ELISA DetectX Progesterone Kit is designed to quantitatively measure progesterone present in dried stool samples, urine, and tissue culture media samples. A progesterone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies.
A progesterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to progesterone in each well. After 2 hours of incubation, the plate is washed and the substrate is added.
The substrate reacts with the bound progesterone-peroxidase DetectX Kit conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring a wavelength of 450 nm.