Tissue-Bank Blog Detectx progesterone Elisa kit

Detectx progesterone Elisa kit

The ELISA DetectX Progesterone Kit is designed to quantitatively measure progesterone present in dried stool samples, urine, and tissue culture media samples. A progesterone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies.

A progesterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to progesterone in each well. After 2 hours of incubation, the plate is washed and the substrate is added.

The substrate reacts with the bound progesterone-peroxidase DetectX Kit conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring a wavelength of 450 nm.

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